The process of cutaneous leishmaniasis is characterized by a sluggish, long-term course due to weak local anti-infective immunity. Therefore, we tried to stimulate local immunity using the official gramicidin paste. To assess the contribution of the antibiotic action of gramicidin S, monomycin ointment was used in one of the control groups as the most effective anti-leishmania drug among the antibiotic agents used to treat cutaneous leishmaniasis.